The Science
The life-changing science behind Prolon
At Prolon, science isn't a tagline; it's our DNA. Developed in collaboration with the Longevity Institute at USC and created by world-renowned longevity scientist Dr. Valter Longo, Prolon's Fasting Mimicking Diet (FMD) has been validated through 25 years of research and 40+ clinical trials.
Supported by $48M in funding and validated by research with 18 leading institutions — including Stanford, Mayo Clinic, the University of Miami, and the University of Genoa — Prolon is the first and only patented nutrition program shown to support longevity through cellular renewal.
Founded by scientists and grounded in peer-reviewed evidence, Prolon brings biotech-grade rigor to everyday nutrition. It's not a trend. It's a proven intervention designed to help you live healthier, longer.
Most read articles and studies
Prolon is the first nutrition program clinically proven to trigger autophagy in humans
A landmark human trial finds the Fasting Mimicking Diet enhances autophagy while improving metabolic health.
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How protein may accelerate your aging — and what you can do about it
High-protein diets are trending, but decades of research show that too much protein — especially from certain sources — can accelerate aging and raise long-term health risks.
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Could you live longer and healthier by reducing your biological age?
Aging is inevitable — but emerging science suggests you may be able to influence your biological age and how well you age over time.
Read more →Explore the research
With more than 40 clinical trials published in leading scientific and medical journals worldwide, Prolon's impact is proven by some of the most rigorous nutrition research to date. Browse a selection of our studies below.
The FMD is the only nutrition program supported by preliminary data confirming autophagy activation in humans GeroScience
A kidney-specific fasting-mimicking diet induces podocyte reprogramming and restores renal function in glomerulopathy Science Translational Medicine
Fasting-mimicking diet causes hepatic and blood markers changes indicating reduced biological age and disease risk Nature Communications
Fasting-mimicking diet and markers/risk factors for aging, diabetes, cancer, and cardiovascular disease: controlled trial Science Translational Medicine
Fasting-Mimicking-Diet does not reduce skeletal muscle function in healthy young adults: a randomized control trial European Journal of Applied Physiology
A Periodic Diet that Mimics Fasting Promotes Multi-System Regeneration, Enhanced Cognitive Performance, and Healthspan Cell Metabolism
The Effects of a Fasting Mimicking Diet on Skin Hydration, Skin Texture, and Skin Assessment: A Randomized Controlled Trial Journal of Clinical Medicine
Fasting Mimicking Diet Cycles vs a Mediterranean Diet and Cardiometabolic Risk in Overweight and Obese Hypertensive Subjects: A Randomized Clinical Trial Nature
Efficacy of a fasting-mimicking diet in functional therapy for depression: A randomised controlled pilot trial
Association of Animal and Plant Protein Intake With All-Cause and Cause-Specific Mortality
An Intermittent Fasting Mimicking Nutrition Bar Extends Physiologic Ketosis in Time Restricted Eating: A Randomized, Controlled, Parallel-Arm Study
Association between IGF-1 levels ranges and all-cause mortality: A meta-analysis
Fasting-mimicking diet prevents high-fat diet effect on cardiometabolic risk and lifespan
Fasting and cancer treatment in humans: A case series report Aging
Fasting-mimicking diet and hormone therapy induce breast cancer regression Nature
Nutrition and fasting mimicking diets in the prevention and treatment of autoimmune diseases and immunosenescence Molecular and Cellular Endocrinology
Fasting-Mimicking Diet Modulates Microbiota and Promotes Intestinal Regeneration to Reduce Inflammatory Bowel Disease Pathology Cell Reports
Fasting-Mimicking Diet Reduces HO-1 to Promote T Cell-Mediated Tumor Cytotoxicity Cancer Cell
Prolonged Fasting reduces IGF-1/PKA to promote hematopoietic stem cell-based regeneration and reverse immunosuppression Cell Stem Cell
A Diet Mimicking Fasting Promotes Regeneration and Reduces Autoimmunity and Multiple Sclerosis Symptoms Cell Reports
Integration of a fasting-mimicking diet programme in primary care for type 2 diabetes reduces the need for medication and improves glycaemic control
From the pioneers in longevity science
Prolon is the only nutrition program patented for supporting longevity, healthspan, and protecting lean body mass through cellular rejuvenation.
Years of scientific research
Decades of studies on longevity and nutrition
Clinical trials
Conducted with world-leading institutions
Leading research institutions
Including USC, Stanford, Mayo Clinic, and more
Pending or issued patents
For optimizing longevity and healthspan
Free download
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Discover the science of the Fasting Mimicking Diet and how it supports cellular renewal, healthy aging, and metabolic health — straight from the pioneers of longevity research.
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